全文获取类型
收费全文 | 26325篇 |
免费 | 1705篇 |
国内免费 | 29篇 |
出版年
2023年 | 192篇 |
2022年 | 236篇 |
2021年 | 792篇 |
2020年 | 486篇 |
2019年 | 533篇 |
2018年 | 754篇 |
2017年 | 704篇 |
2016年 | 943篇 |
2015年 | 1133篇 |
2014年 | 1414篇 |
2013年 | 1963篇 |
2012年 | 2043篇 |
2011年 | 1900篇 |
2010年 | 1172篇 |
2009年 | 1031篇 |
2008年 | 1256篇 |
2007年 | 1210篇 |
2006年 | 1025篇 |
2005年 | 965篇 |
2004年 | 843篇 |
2003年 | 730篇 |
2002年 | 697篇 |
2001年 | 463篇 |
2000年 | 473篇 |
1999年 | 400篇 |
1998年 | 207篇 |
1997年 | 166篇 |
1996年 | 145篇 |
1995年 | 161篇 |
1994年 | 136篇 |
1993年 | 108篇 |
1992年 | 270篇 |
1991年 | 242篇 |
1990年 | 228篇 |
1989年 | 173篇 |
1988年 | 251篇 |
1987年 | 185篇 |
1986年 | 162篇 |
1985年 | 189篇 |
1984年 | 161篇 |
1983年 | 126篇 |
1982年 | 133篇 |
1981年 | 105篇 |
1980年 | 103篇 |
1979年 | 123篇 |
1978年 | 103篇 |
1977年 | 118篇 |
1976年 | 103篇 |
1975年 | 95篇 |
1974年 | 100篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
31.
32.
33.
34.
35.
36.
Abstract The bifunctional T-protein (chorismate mutase-T: cyclohexadienyl dehydrogenase) of l -tyrosine biosynthesis was found to be present in all genera making up the enteric bacteria. The dehydrogenase component of the T-protein was active with both prephenate and l -arogenate, showing it to be a cyclohexadienyl dehydrogenase. The dehydrogenase component, but not the mutase component, of the T-protein was feedback-inhibited by l -tyrosine. Unlike some other bifunctional proteins, the T-protein has evolved recently and is not ubiquitous. However, once the biochemical specialization of bifunctionality becomes established, the results indicate that such character states are strongly conserved through evolutionary time. Thus, bifunctional proteins can provide particularly reliable markers for small (recent origin), intermediate, and large (ancient origin) phylogenetic clusters. 相似文献
37.
Siddhartha Kumar Bhaumik Manoj Kumar Singh Subir Karmakar Tripti De 《Glycoconjugate journal》2009,26(6):663-673
As compared to cutaneous leishmaniasis, vaccination against visceral leishmaniasis (VL) has received limited attention. In
this study, we demonstrate for the first time that an UDP-Galactose: N-acetylglucosamine β 1–4 galactosyltransferase (GenBank Accession No. EF159943) expressing attenuated LD clonal population (A-LD) is able to confer protection against the experimental challenge with the virulent LD AG83 parasite. A-LD was also effective in established leishmania infection. The vaccinated animals showed both cell mediated
(in vitro T-cell proliferation, and DTH response) and humoral responses (Th1 type). These results demonstrate the potential of the
attenuated clones as an immunotherapeutic and immunoprophylactic agent against visceral leishmaniasis. 相似文献
38.
Experimental evidence suggests that proteins adsorbed to hydrophobic surfaces at low coverages are stabilized relative to the bulk. For larger coverages, proteins unfold and form β-sheets. We performed computer simulations on model proteins and found that: 1), For weakly adsorbing surfaces, unfolded conformations lose more entropy upon adsorption than folded ones. 2), The melting temperature, both in the bulk and at surfaces, decreases with increasing protein concentration because of favorable interprotein interactions. 3), Proteins in the bulk show large unfolding free energy barriers; this barrier decreases at stronger adsorbing surfaces. We conjecture that typical experimental temperatures appear to be below the bulk melting temperature for a single protein, but above the melting temperature for concentrated protein solutions. Purely thermodynamic factors then explain protein stabilization on adsorption at low concentrations. However, both thermodynamic and kinetic factors are important at higher concentrations. Thus, proteins in the bulk do not denature with increasing concentration due to large kinetic barriers, even though the aggregated state is thermodynamically preferred. However, they readily unfold upon adsorption, with the surface acting as a heterogeneous catalyst. The thermal behavior of proteins adsorbed to hydrophobic surfaces thus appears to follow behavior independent of their chemical specificity. 相似文献
39.
Eriocaulon raipurense (Eriocaulaceae) is described and illustrated as a new species from Madhya Pradesh, India. The species is closely allied to E. hamiltonianum but differs in the size and apex of involucral bancts, white-pilose nature of floral bracts and colour of female petals. 相似文献
40.
Marjorie Jones Roy W. Keenan 《Biochimica et Biophysica Acta (BBA)/General Subjects》1981,678(3):403-407
A procedure was developed for the detection of 2′,3′-cyclic nucleotide 3′-phosphohydrolase in myelin. This assay was sufficiently sensitive to detect the low levels of 2′,3′-cyclic nucleotide 3′-phosphohydrolase in human erythrocytes. The 2′,3′-cyclic nucleotide 3′-phosphohydrolase of human erythrocytes was determined to be exclusively associated with the inner (cytosolic) side of the membrane. Leaky ghostsand resealed ghosts were assayed for 2′,3′-cyclic nucleotide 3′-phosphohydrolase, (Ca2+/Mg2+-ATPase, and acetylcholinesterase activity, and the 2′,3′-cyclic nucleotide 3′-phosphohydrolase profile is the same as that of the (Ca2+/Mg2+)-ATPase, an established inner membrane maker. 相似文献